Journal: Scientific Reports

Article Title: Functional and molecular single-cell analyses implicate PRDM14 in the initiation of B cell leukemia in mice

doi: 10.1038/s41598-025-93043-z

Figure Lengend Snippet: Pseudotime analysis identifies regulators of differentiation, including AP-1 genes, that play a role in pre-leukemic B-1-cell identity. ( A ) B cell-specified common lymphoid progenitors (CLPs) progress through multiple stages of development to become immature B cells after undergoing V(D)J antigen receptor heavy (H) and light (L) chain recombination. B-2 cell development occurs in the bone marrow, whereas B-1 cell development occurs in the fetal liver. Early B-1 progenitor cells have been identified in the yolk sac as early as E8.25. Immature B-1 cells differentiate into CD5 + B-1a or CD5- B-1b cells in the periphery, predominantly in serous cavities (e.g. pleural, peritoneal cavities). Expression of defining cell surface markers are indicated below each cell type. Cell surface markers expressed during B-1 cell development are not well defined. sIgM, surface immunoglobulin M. Created with BioRender.com. ( B ) B-1 (left) or B-2 (right) signature gene sets from Fitch et al. . Expression of signature genes in scRNA-seq data from Prdm14 -expressing pre-leukemic B cells is indicated, with significant differentially expressed genes highlighted in green (up-regulated) or orange (down-regulated). ( C ) Gene Ontology analysis of down-regulated genes in sorted pre-leukemia cells relative to immunophenotype-matched controls. ( D ) Pseudotime analysis was restricted to one manifold which included B cell-associated clusters 0, 1, 2, 11, and 17. One lineage was identified, beginning at cluster 11 and ending at cluster 1. ( E ) Thirty transcription factors were significantly differentially expressed along the pseudotime axis. ( F ) GO analysis of the 30 transcription factors identified enrichment of various biological processes.

Article Snippet: CD5-APC , eBioscience , 53 − 7.3.

Techniques: Expressing

Journal: Scientific Reports

Article Title: Functional and molecular single-cell analyses implicate PRDM14 in the initiation of B cell leukemia in mice

doi: 10.1038/s41598-025-93043-z

Figure Lengend Snippet: Antibodies used for the CyTOF experiments.

Article Snippet: CD5-APC , eBioscience , 53 − 7.3.

Techniques:

Journal: Scientific Reports

Article Title: Functional and molecular single-cell analyses implicate PRDM14 in the initiation of B cell leukemia in mice

doi: 10.1038/s41598-025-93043-z

Figure Lengend Snippet: Antibodies used for the flow cytometry experiments.

Article Snippet: CD5-APC , eBioscience , 53 − 7.3.

Techniques: Flow Cytometry

Journal: STAR Protocols

Article Title: Protocol to induce cell labeling in vivo and to trace labeled hematopoietic cells using mouse models

doi: 10.1016/j.xpro.2024.103408

Figure Lengend Snippet:

Article Snippet: CD5 53-7.3 , Tonbo , 20-0051.

Techniques: Recombinant

Journal: STAR Protocols

Article Title: Protocol to induce cell labeling in vivo and to trace labeled hematopoietic cells using mouse models

doi: 10.1016/j.xpro.2024.103408

Figure Lengend Snippet: Examples: Staining 5 samples of peritoneal cells for B-1 cell identification

Article Snippet: CD5 53-7.3 , Tonbo , 20-0051.

Techniques: Staining

Journal: iScience

Article Title: Arginine methylation of the p30 C/EBPα oncoprotein regulates progenitor proliferation and myeloid differentiation

doi: 10.1016/j.isci.2024.111199

Figure Lengend Snippet:

Article Snippet: CD5 APC (Lineage cocktail) , eBioscience , #17-0051-82.

Techniques: Magnetic Beads, Virus, Retroviral, Recombinant, Red Blood Cell Lysis, Mutagenesis, Transformation Assay, Derivative Assay, Plasmid Preparation, Software, Imaging